Anonymous User As Domain Administrator

I have several website that use asp pages and backend databases running on my win2000 server. I removed everyone from the permissions on all of my Hard drives and added the annonymous user to the winnt folder and the wwwroot folder and all of the folders within these directories. Now my sites that just run straight html work fine but my sites with .asp pages are having problems.

However if I add the Domain Administrator account as the annonymous user to the site it will work. Then I have reverted back to the I_USR account and the site still works until the server has to be rebooted. At this point the site won't work until I add and then remove the admin user as the annonymous user to the site.